htsjdk.samtools.SAMFormatException

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    • htsjdk.samtools.SAMFormatException: SAM validation error: ERROR: Record 40060, Read name ERR001301.6170436, bin field of BAM record does not equal value computed based on alignment start and end, and length of sequence to which read is aligned at htsjdk.samtools.SAMUtils.processValidationErrors(SAMUtils.java:441) at htsjdk.samtools.BAMFileReader$BAMFileIterator.advance(BAMFileReader.java:652) at htsjdk.samtools.BAMFileReader$BAMFileIterator.next(BAMFileReader.java:637) at htsjdk.samtools.BAMFileReader$BAMFileIterator.next(BAMFileReader.java:607) at htsjdk.samtools.SamReader$AssertingIterator.next(SamReader.java:545) at htsjdk.samtools.SamReader$AssertingIterator.next(SamReader.java:519) at picard.sam.SamToFastq.doWork(SamToFastq.java:174) at picard.cmdline.CommandLineProgram.instanceMain(CommandLineProgram.java:209) at picard.cmdline.PicardCommandLine.instanceMain(PicardCommandLine.java:95) at picard.cmdline.PicardCommandLine.main(PicardCommandLine.java:105)
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