java.lang.IllegalArgumentException: start must be less than or equal to end!

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    Root Cause Analysis

    1. java.lang.IllegalArgumentException

      start must be less than or equal to end!

      at net.sf.picard.util.Interval.<init>()
    2. picard
      Interval.<init>
      1. net.sf.picard.util.Interval.<init>(Interval.java:74)
      2. net.sf.picard.util.Interval.<init>(Interval.java:52)
      2 frames
    3. org.bioinfo.ngs
      NgsSmartMain.main
      1. org.bioinfo.ngs.qc.qualimap.common.TranscriptDataHandler.createHelperMaps(TranscriptDataHandler.java:674)
      2. org.bioinfo.ngs.qc.qualimap.common.TranscriptDataHandler.constructTranscriptsMap(TranscriptDataHandler.java:174)
      3. org.bioinfo.ngs.qc.qualimap.process.ComputeCountsTask.loadRegionsFromGTF(ComputeCountsTask.java:565)
      4. org.bioinfo.ngs.qc.qualimap.process.ComputeCountsTask.initRegions(ComputeCountsTask.java:474)
      5. org.bioinfo.ngs.qc.qualimap.process.ComputeCountsTask.run(ComputeCountsTask.java:383)
      6. org.bioinfo.ngs.qc.qualimap.process.RNASeqQCAnalysis.run(RNASeqQCAnalysis.java:67)
      7. org.bioinfo.ngs.qc.qualimap.main.RnaSeqQcTool.execute(RnaSeqQcTool.java:135)
      8. org.bioinfo.ngs.qc.qualimap.main.NgsSmartTool.run(NgsSmartTool.java:187)
      9. org.bioinfo.ngs.qc.qualimap.main.NgsSmartMain.main(NgsSmartMain.java:103)
      9 frames